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ROTIGROW ONESTEP™
ROTIGROW ONESTEP™
A frozen blend of 7 microalgae for maximum Rotifer Production with full Rotifer Enrichment. High in carotenoids and phospholipids. 2-year frozen shelf life with a 3-week refrigerated shelf life.
Product Description
Applications & Benefits
Directions & Feeding
Storage & Technical Data
Product Description
Applications & Benefits
Directions & Feeding
Storage & Technical Data
Product Description
RotiGrow OneStep™ is a frozen blend of 7 microalgae for maximum Rotifer Production with full Rotifer Enrichment. High in carotenoids and phospholipids. 2-year frozen shelf life with a 3-week refrigerated shelf life.
Frozen Microalgal Blend for Rotifer Production with Full Rotifer Enrichment
Applications & Benefits
Directions & Feeding
Directions
- Defrost frozen Rotigrow OneStep slowly in a refrigerator overnight. Rotigrow OneStep can be poured directly into the larval tank, but pre-diluting. (Note: 10 liter Cubitainers and cases may require 2 days defrosting)
- Mixing and screening are not necessary. DO NOT BLEND; it is unnecessary and may damage the algae cells.
- Feed according to the table below. Adjust dosage to meet the needs of your hatchery and larvae
Typical Feeding Protocol
Rotifer Production
* Feed rates based on 165micron l-type rotifer with a mass of 0.18g dry weight/million rotifers
Continuous
- Feed rate: 2ml/million rotifers – use rotifer count after harvest
- Typical harvest is 35-40% per day
- Feed 4-6 times per day
- Feed continuously for best results
- Divide daily feed rate by number of feedings
- If you harvest more or less rotifers per day, adjust feeding according to the table below
| Harvest Rate | Feed / million rotifers (ml) | Rotifers produced per liter Rotigrow Nanno |
|---|---|---|
| 25% | 1.2 | 320 |
| 35% | 1.9 | 320 |
| 50% | 3.4 | 320 |
Batch Production
- Feed rate: 2ml/million rotifers
- Feed 4-6 times per day or continuously
- Divide daily feed rate by number of feedings
- Feed continuously for best results
- For faster or slower growth rate, use table below
Batch Production Systems
| Rotifer Density (per ml) | ||||||
|---|---|---|---|---|---|---|
| Rotigrow OneStep (feed per day per million rotifers) |
Day 0 | Day 1 | Day 2 | Day 3 | Rotifer Harvest (per liter of feed) |
Growth Rate (per day) |
| 1.4 ml | 500 | 706 | 996 | 1406 | 320 million | 41% |
| 2.0 ml | 500 | 795 | 1264 | 2010 | 320 million | 59% |
| 2.9 ml | 500 | 9910 | 1656 | 3013 | 320 million | 82% |
Nutritional Profile of Harvested Rotifers
| Rotigrow OneStep | |
|---|---|
| Protein | 67% |
| Lipids | 11.3% |
| DHA | 26.5 mg/g * |
| EPA | 8.0 mg/g * |
| ARA | 5.7 mg/g * |
| HUFAs | 50 mg/g * |
| Polar Lipids | 55% |
| Sterols | 4.4% |
| Free Fatty Acids | 5% |
| Triglicerides | 35% |
* dry weight
Storage & Technical Data
| Packaging | 1-quart bottle, 1-liter bag, 10-liter cubitaner® |
|---|---|
| Storage | 2 years shelf life when frozen 3 week refrigerated shelf life after defrosting |
| Tip | If thawed and won't be used within 12 to 14 weeks, freeze in standard ice cube for 24 hours Store frozen cubes in plastic bag in freezer and then thaw before use |
| Physical Properties | |
|---|---|
| Appearance | Viscous green or brown Liquid |
| Algal cell size | 1.5 - 12.0 microns |
| Cell Density | Billion/ml |
| Algal Biomass | 148g Dry biomass/L |
| Composition of Dry Algal Biomass | |
|---|---|
| Protein | 67.0% |
| Lipid | 11.3% |
| EPA (% of Lipids) | 9.3% |
| DHA (% of Lipids) | 21.5% |
| ARA (% of Lipids) | 1.1% |
| Carbohydrate | 19.0% |
| Ash | 3.5% |
| Composition of Liquid Product | |
|---|---|
| Protein | > 8.4% |
| Lipids | > 2.8% |
| Carbohydrate | > 2.8% |
| Ash | < 5.0% |
| Moisture | < 81% |
| Microbial Specification | |
|---|---|
| Coliform Bacteria | 2 <0.3 mpn/mL |
| Salmonella | Negative |
| Known pathogenic marine bacteria | Negative |