TP 3000
A frozen, whole-cell concentrate of Thalassiosira pseudonan — a nutritionally balanced and very clean microalgae for all live stages from larvae to broodstock
Product Description
Applications & Benefits
Directions & Feeding
Directions
- Defrost TP 3000 overnight in a refrigerator.
- When water is circulated with a centrifugal pump, such as with an upweller silo, TP 3000 can be added in front of the pump intake and the pump will disperse the algae cells.
- When feeding into open tanks or header tanks, pre-dilute the algae 10:1 with system water.
- Add system water to a small bucket.
- Add TP 3000 and stir.
- DO NOT BLEND; it is unnecessary and may damage the algae cells.
- Pour into larvae tank or spat.
- Pouring through a 20–micron screen can improve dispersion.
- Feed according to the table below. Adjust dosage to meet the needs of your hatchery and larvae.
- Refrigerate bottle when not in use.
- Use within 8 days of defrosting.
Typical Feeding Ration
Larvae
Feed according to shell length. Shell length on a given day is an estimate. Larviculture may run up to 20 days.
Daily Feed Rates per million larvae, for Crassostrea virginica (from FAO protocol)
| Day | Mean shell length | TP 3000 (ml) | Liters of live Isochrysis (4x10^6 cells/ml) |
|
|---|---|---|---|---|
| 2 | 75 | 0.3 | = | 0.4 |
| 3 | 95 | 0.4 | = | 0.6 |
| 4 | 100 | 0.5 | = | 0.7 |
| 5 | 115 | 1.6 | = | 2.1 |
| 6 | 130 | 2.7 | = | 3.6 |
| 7 | 145 | 3.9 | = | 5.1 |
| 8 | 160 | 4.9 | = | 6.6 |
| 9 | 190 | 7.1 | = | 9.5 |
| 10 | 220 | 9.3 | = | 12.4 |
| 11 | 240 | 10.9 | = | 14.4 |
| 12 | 260 | 12.2 | = | 16.3 |
| 13 | 270 | 13.0 | = | 17.3 |
| 14 | 280 | 13.7 | = | 18.3 |
Spat
Feed By Live Weight
- Clean and weigh the seed
- 0.4ml TP 3000 per gram live weight of spat per day
- Divide total daily feed among 3 or more feedings per day
- Works for all sizes of spat
- Clean seed, weigh and adjust feed rate frequently
– OR –
Feed by Seed Count and Size
(Feeding 1 million Oyster Spat; based on FAO manual, p. 137, Table 14)
| Length (mm) | TP 3000 feed per day |
|---|---|
| 0.3 | 5 ml |
| 0.5 | 36 ml |
| 1.0 | 153 ml |
| 2.0 | 1.2 Liters |
| 3.0 | 3.5 Liters |
Feeding Broodstock Conditioning and Fattening
Typical Broodstock Feed Rates:
0.022 – 0.043 ml TP 3000 per gram wet meat weight per day
-OR-
0.2 – 0.7 ml TP 3000 per adult animal per day (depending on feed ration and size*)
*The required food ration for conditioning is based on the meat weight of the adults. It is usually between 2 and 4% of the mean dry meat in dry weight of algae fed per day. This is approximately equivalent to 0.2 – 0.7 ml TP 3000 per gram of wet meat weight.
Storage & Technical Data
| Packaging | 1 liter bottle |
|---|---|
| Storage | 2-year shelf life when frozen Use within 8 days after defrosting |
| Tip | Keep frozen before defrosting, refrigerate after defrosting |
| Physical Properties | |
|---|---|
| Description | 100% Thalassiosira pseudonana |
| Appearance | Viscous Brown Concentrate |
| Cell Size | 4.5 – 7 microns |
| Cell Density | ~2.1 Billion cells per ml |
| Algal Biomass | 150g Dry biomass/L |
| Composition of Dry Algal Biomass (Typical) | |
|---|---|
| Protein | 45% |
| Lipids | 10% |
| DHA (% of lipids) | 9.3% |
| EPA (% of lipids) | 20.5% |
| Carbohydrate | 12.0% |
| Ash | 33% |
| Guaranteed Composition of Frozen Product | |
|---|---|
| Protein | > 6.7% |
| Lipids | > 1.5% |
| Carbohydrate | > 1.8% |
| Ash | < 8.5% |
| Moisture | < 85% |
| Microbial Specification | |
|---|---|
| Coliform Bacteria | 2 <0.3 mpn/mL |
| Salmonella | Negative |
| Known pathogenic marine bacteria | Negative |