Nanno 3600 (Nannochloropsis)
High Yield Grow-out Feed
Nanno 3600 is our original high-yield rotifer feed. It is single-species (Nannochloropsis) and produces phospholipid-rich rotifers. It also provides a high Feed Conversion Rate with minimal organic waste in the tank, and gives an EPA and ARA pre-enrichment boost for use with high-DHA enrichment protocols. Strain: CCMP525
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NOTE: For new customers, we suggest our next-generation product, RotiGrow Nanno
Applications: Finfish Hatcheries Shellfish Hatcheries
Related Products: Instant Algae® Rotigrow® N-Rich® Rotigreen®
Nanno 3600 Offers:
- Healthy Rotifer Cultures — Rapid growth, minimal fouling and reduced bacteria loads
- High Yields — Rotifers can double daily at densities over 8,000 L-Type rotifers per ml
- Optimum Enrichment — Supports full highly-unsaturated fatty acid (HUFA) enrichment; the best source of EPA and ARA as well as critical vitamins
- Concentrated, Clean, Liquid Form — Exceptionally clean and easy to use with minimal foaming or clumping, which means less time spent maintaining cultures
- Long shelf life — frozen, three years
Storage: Instant Algae Nannochloropsis can be stored in a refrigerator for 3 months (best if stored at -1 to +3C) or it can be frozen for longer shelf life (3 years). When frozen the algae will "hard-freeze" like ice.
Ice Cubes: If you have received a liter of Nanno and don't plan to use it all within 3 months of thawing a frozen bag, we suggest making it into ice cubes. Simply pour the algae into a standard ice cube tray and freeze for 24 hours. Then take the cubes and store them in a plastic bag in your freezer. As you need more algae, remove a cube from the plastic bag and thaw.
Product Description
| Description | Concentrated Blend of Microalgae |
|---|---|
| Appearance | Green Viscous Liquid |
| Algal size | 1.5-2.0 microns |
| Typical Cell Count (see Dry Weight vs Cell Count)* | 68 billion/ml |
| Live Algae Equivalent | 3600 liters @ 19 million cells/ml |
| Type | Non-flagellated Yellow-Green Eustigmatophyceae |
| Dry Weight | >18% |
| Shelf Life | 12-14 weeks 3 years frozen |
Composition of Biomass (dry wt)
| Protein | 58.6% |
|---|---|
| Lipid | 14.5% |
| Carbohydrate | 20.0% |
| Ash | 5.9% |
Feed and Harvest Rates
Continuous Production Systems
| Harvest Rate | 20% | 30% | 35% | 40% | 45% |
|---|---|---|---|---|---|
| Nanno 3600 Feed Rate (ml per day/million rotifers) | 0.63 | 0.94 | 1.09 | 1.25 | 1.41 |
| Rotifer Harvest (million rotifers per liter of feed) | 320 | 320 | 320 | 320 | 320 |
NOTES: 1. Feed rates based on rotifer count just prior to harvest
2. For 165 micron long L-Type rotifers with a mass of 0.18g dry weight/million rotifers
3. Feed continuously for best results
Batch Production Systems
| Rotifer density (per ml) | ||||||
|---|---|---|---|---|---|---|
| Nanno 3600 (feed per day per million rotifers) | Day 0 | Day 1 | Day 2 | Day 3 | Rotifer Harvest (per liter of feed) | Growth Rate (per day) |
| 1.3 ml | 500 | 706 | 996 | 1,406 | 320 million | 41% |
| 1.8 ml | 500 | 795 | 1,264 | 2,010 | 320 million | 59% |
| 2.6 ml | 500 | 910 | 1,656 | 3,013 | 320 million | 82% |
NOTES: 1. Feed rates based on rotifer counts at the beginning of the day
2. For 165 micron long L-Type rotifers with a mass of 0.18g dry weight/million rotifers
3. Feed days 0, 1 and 2. Feed continuously for best results
Fatty Acids
| 12:0 | Lauric acid | 0.0 | 18:3n6 | GLA | 0.6 |
| 14:0 | Myristic acid | 2.2 | 18:3n3 | ALA | 0.3 |
| 16:0 | Palmitic acid | 21.5 | 18:4n3 | SDA | 0.1 |
| 16:1n7 | Palmitoleic acid | 3.3 | 20:3n6 | DGLA | 0.3 |
| 16:2n4 | Hexadecadienoic | 0.2 | 20:4n6 | ARA | 3.6 |
| 18:0 | Stearic acid | 0.4 | 20:5n3 | EPA | 30.3 |
| 18:1n9 | Oleic acid | 18.0 | 22:5n3 | DPA (n-3) | 0.0 |
| 18:2n6 | LA | 4.7 | 22:6n3 | DHA | 0.0 |
NOTE: fatty acid units after "profile" units are mg/g d.w.
Summary
| DHA : EPA : ARA | 0 : 1.0 : 0.12 |
|---|---|
| EPA + DHA | 30 mg/g d.w. (Biomass) |
| Total HUFAs, 20:3n3 | 35 mg/g d.w. (Biomass) |
| Total fatty acids | 105 mg/g d.w. (Biomass) |
Rotifer Composition
Essential Fatty Acids and Total Lipids
Typical profiles of rotifers grown on Nanno 3600 and enriched using N-Rich High PRO, a protein-rich feed, and N-Rich PL Plus, a phospholipid-rich feed.
| Nutrient | Nanno 3600 | 3-hour enrichment N-Rich PL Plus | 6 to 8 hour enrichment N-Rich PL Plus & High PRO | Cod eggs | Cobia eggs |
|---|---|---|---|---|---|
| Protein | 68% | 66% | 64% | 79% | n/a |
| DHA | 0.0 | 30 | 38 | 33 | 42 |
| EPA | 20 | 13 | 12 | 18.6 | 13.5 |
| ARA | 2.8 | 2.5 | 2.5 | 2.2 | 3.4 |
| HUFAs | 32 | 50 | 57 | 56.2 | 68 |
| Total fatty acids | 8.9% | 13.0% | 14.0% | 13.2% | 22% |
| Polar lipids | 56% | 55% | 55% | 71% | n/a |
| Sterols | 5% | 4.2% | 4.3% | 9.8 | n/a |
| Free fatty acids | 5% | 5.1% | 4.9% | 5.4 | n/a |
NOTE: fatty acid units after "profile" units are mg/g