RotiGrow® Plus
All-in-One Grow-Out Feed with Omega Pre-Enrichment
RotiGrow Plus is a clean, high yield rotifer feed that maximizes pre-enrichment levels of DHA, EPA and ARA. It is the essential first step in the RotiGrow System. It can be used as a stand-alone feed depending on the nutritional requirements of the fish larvae, or in combination with one of our N-Rich enrichment products.
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Applications: Finfish Hatcheries Shellfish Hatcheries
Related Products: Instant Algae® Rotigrow® N-Rich® Rotigreen®
Photomicrograph of RotiGrow Plus
RotiGrow Plus Offers:
- Healthy Rotifer Cultures — Rapid growth, minimal fouling and reduced bacteria loads
- Higher Yields — Rotifers can double daily at densities over 5,000 L-Type rotifers per ml
- Essential Omega Boost — Provides 50-100 percent of the Omega-3 fatty acids needed by the larvae of most marine species — without secondary enrichment!*
- Optimum Enrichment — Supports full highly-unsaturated fatty acid (HUFA), full vitamin enrichment with proper protocols, and additional carotenoid enrichment of rotifers without sacrificing other nutritional factors or stressing the rotifers
- Concentrated, Clean, Liquid Form — Exceptionally clean and easy to use with minimal foaming or clumping, which means less time spent maintaining cultures
- All-In-One Feed — For species such as barramundi, sea bass, zebra fish and many ornamental fish such as clown fish, RotiGrow Plus is an excellent “all-in-one” feed and enrichment product**
* Meets Cod Egg profile for Omega-3 fatty acids when using Steps One and Two
** Other fish such as cobia, cod, sea bream seriola, flounder, drum, and grouper
require the supplemental enrichment of our N-Rich products
The Rotifer Instant Algae System
RotiGrow Plus is part of a complete line of blended concentrate Instant Algae products to support optimal grow-out, enrichment and productivity for rotifers and larval fish. No other feed is closer to nature or better for your rotifer, fish and hatchery productivity.
Standard System:
STEP 1: Grow-out: RotiGrow Plus – Grow-Out Feed and Pre-Enrichment
STEP 2: Enrich: N-Rich – one to eight-hour enrichments
STEP 3: Cold store and gut load: N-Rich High PRO provides digestive and nutritional support
STEP 4: Greenwater: RotiGreen, RotiGreen Nanno, or RotiGreen Iso – supports health of rotifers and fish
System for barramundi, sea bass, zebra fish and many ornamental fish such as clown fish:
STEP 1: RotiGrow Plus – All-in-One Feed, Pre-Enrichment and Enrichment
STEP 2: RotiGreen, RotiGreen Nanno, or RotiGreen Iso – supports health of rotifers and fish
Custom RotiGrow Blends Available
Custom blends can be designed for slow enrichment, fast enrichment, and achievement of HUFA levels above 8% dry-weight rotifer biomass. Please contact us for more information.
Product Description
| Description | Concentrated Blend of Microalgae |
|---|---|
| Appearance | Green Viscous Liquid |
| Algal size | 1.5-12 microns |
| Dry Weight | >14.8% |
Composition of Biomass (dry wt)
| Protein | 55.7% |
|---|---|
| Lipid | 19.5% |
| Carbohydrate | 19.0% |
| Ash | 5.8% |
Feed and Harvest Rates
Continuous Production Systems
| Harvest Rate | 20% | 30% | 35% | 40% | 45% |
|---|---|---|---|---|---|
| RotiGrow Plus Feed Rate (ml per day/million rotifers) |
0.75 | 1.13 | 1.32 | 1.57 | 1.70 |
| Rotifer Harvest (million rotifers per liter of feed) | 265 | 265 | 265 | 265 | 265 |
NOTES: 1. Feed rates based on rotifer count just prior to harvest
2. For 165 micron long L-Type rotifers with a mass of 0.18g dry weight/million rotifers
3. Feed continuously for best results
Batch Production Systems
| Rotifer density (per ml) | ||||||
|---|---|---|---|---|---|---|
| RotiGrow Plus (feed per day per million rotifers) | Day 0 | Day 1 | Day 2 | Day 3 | Rotifer Harvest (per liter of feed) | Growth Rate (per day) |
| 1.3 ml | 500 | 706 | 996 | 1,406 | 265 million | 41% |
| 1.8 ml | 500 | 795 | 1,264 | 2,010 | 265 million | 59% |
| 2.6 ml | 500 | 910 | 1,656 | 3,013 | 265 million | 82% |
NOTES: 1. Feed rates based on rotifer counts at the beginning of the day
2. For 165 micron long L-Type rotifers with a mass of 0.18g dry weight/million rotifers
3. Feed days 0, 1 and 2. Feed continuously for best results
Fatty Acids
| 12:0 | Lauric acid | 3.0 | 18:3n6 | GLA | 2.9 |
| 14:0 | Myristic acid | 11.4 | 18:3n3 | ALA | 0.4 |
| 16:0 | Palmitic acid | 24.4 | 18:4n3 | SDA | 5.4 |
| 16:1n7 | Palmitoleic acid | 11.7 | 20:3n6 | DGLA | 0.2 |
| 16:2n4 | Hexadecadienoic | 3.4 | 20:4n6 | ARA | 2.1 |
| 18:0 | Stearic acid | 1.4 | 20:5n3 | EPA | 18.1 |
| 18:1n9 | Oleic acid | 11.8 | 22:5n3 | DPA (n-3) | 2.1 |
| 18:2n6 | LA | 10.9 | 22:6n3 | DHA | 41.9 |
NOTE: fatty acid units after "profile" units are mg/g d.w.
Summary
| DHA : EPA : ARA | 2.3 : 1.0 : 0.12 |
|---|---|
| EPA + DHA | 60 mg/g d.w. (Biomass) |
| Total HUFAs, 20:3n3 | 64 mg/g d.w. (Biomass) |
| Total fatty acids | 154 mg/g d.w. (Biomass) |
Rotifer Composition
Essential Fatty Acids and Total Lipids
Typical profiles of rotifers grown on RotiGrow Plus and enriched using N-Rich High PRO, a protein-rich feed, and N-Rich PL Plus, a phospholipid-rich feed.
| Rotigrow Plus | 2-hour enrichment N-Rich High PRO |
8-hour enrichment N-Rich High PRO |
2-hour enrichment N-Rich PL Plus |
Cod eggs | Cobia eggs | |
|---|---|---|---|---|---|---|
| Protein | 68% | 66% | 64% | 65% | 79% | n/a |
| DHA | 17 | 30 | 40 | 36 | 33 | 42 |
| EPA | 12 | 13 | 12 | 10 | 18.6 | 13.5 |
| ARA | 1.9 | 2.5 | 2.0 | 1.9 | 2.2 | 3.4 |
| HUFAs | 43 | 50 | 58 | 51 | 56.2 | 68 |
| Total fatty acids | 10.5% | 13.0% | 14.2% | 12.6% | 13.2% | 22% |
| Polar lipids | 54% | 55% | 55.7% | 55% | 71% | n/a |
| Sterols | 4% | 4.2% | 4.3% | 4.0% | 9.8% | n/a |
| Free fatty acids | 5.9% | 5.1% | 4.9% | 5.0% | 5.4% | n/a |
NOTE: fatty acid units after "profile" units are mg/g