RotiGrow® Nanno (Nannochloropsis)
Highest Yielding Grow-Out Feed
RotiGrow Nanno is our highest yielding rotifer feed. It is single-species (Nannochloropsis) and produces phospholipid-rich rotifers. It also provides the highest Feed Conversion Rate of our products, with the least organic waste in the tank, and gives a high EPA and ARA pre-enrichment boost for use with high DHA-enrichment protocols. Strain: CCMP525
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Applications: Finfish Hatcheries Shellfish Hatcheries
Related Products: Instant Algae® Rotigrow® N-Rich® Rotigreen®
Photomicrograph of RotiGrow Nanno
RotiGrow Nanno Offers:
- Healthy Rotifer Cultures — Rapid growth, minimal fouling and reduced bacteria loads
- Highest Yields — Rotifers can double daily at densities over 8,000 L-Type rotifers per ml
- Optimum Enrichment — Supports full highly-unsaturated fatty acid (HUFA) enrichment; the best source of EPA and ARA as well as critical vitamins
- Concentrated, Clean, Liquid Form — Exceptionally clean and easy to use with minimal foaming or clumping, which means less time spent maintaining cultures
- Long shelf life — frozen, three years
The RotiGrow System *
A two-step rotifer feed and enrichment system employing two supplemental feeds that further enhance larval fish nutrition. All our feeds are produced using proprietary processes with 100 percent of the biomass composed of nutritionally rich blends of microalgae. All feeds contain 75 – 95 percent intact whole cells for whole-food, whole life-cycle support.
STEP 1: RotiGrow Nanno
Highest Yielding Grow-out Feed
Supports Pre-Enrichment and Highest Yields
RotiGrow Nanno, a single-species (Nannochloropsis) feed, provides the highest Feed Conversion Rate of our products, with the least organic waste in the tank, and gives a high EPA and ARA pre-enrichment boost for use with high DHA-enrichment protocols.
STEP 2: N-Rich
One to Eight Hour Secondary Enrichment (with blends to meet the needs of your species of fish)
Full HUFA and vitamin enrichment plus "Whole-Cell" Nutrition
N-Rich offers a unique blend of microalgae that provides a wide spectrum of essential nutrients, including high levels of protein, carotenoids, sterols and optimized vitamin C, A, D and B vitamins. N-Rich can raise the nutritional profile of enriched rotifers to meet the needs of any fish.
Additional Products for Nutrition Enhancements:
STEP 3 (OPTIONAL): Cold storage and/or Gut Loading with N-Rich or Isochrysis/Pavlova Blend
Digestive and "Whole-Cell" Nutritional Support for Larval Fish
N-Rich High PRO is a nutritionally balanced rotifer finishing feed that is easily digested when released into fish larval guts. It contains a blend of microalgae including Pavlova and Isochrysis. N-Rich High PRO offers an optimal fatty acid profile, plus carotenoids, sterols and the right amounts of vitamins A, D, C, and B's.
STEP 4: RotiGreen
Greenwater: Isochrysis, Nannochloropsis and DHA/EPA blends Greenwater to Promote Overall Fish Health
RotiGreen greenwater (RotiGreen Iso, RotiGreen Nanno and RotiGreen Omega) further supports rotifer and larval nutrition in the larval tank to promote health and enhance survival.
Product Description
| Description | Concentrated Blend of Microalgae |
|---|---|
| Appearance | Green Viscous Liquid |
| Algal size | 1.5-2.0 microns |
| Dry Weight | >16.4% |
| Shelf Life | 12-14 weeks 3 years frozen |
Composition of Biomass (dry wt)
| Protein | 58.6% |
|---|---|
| Lipid | 14.5% |
| Carbohydrate | 20.0% |
| Ash | 5.9% |
Feed and Harvest Rates
Continuous Production Systems
| Harvest Rate | 20% | 30% | 35% | 40% | 45% |
|---|---|---|---|---|---|
| Rotigrow Nanno Feed Rate (ml per day/million rotifers) | 0.63 | 0.94 | 1.09 | 1.25 | 1.41 |
| Rotifer Harvest (million rotifers per liter of feed) | 320 | 320 | 320 | 320 | 320 |
NOTES: 1. Feed rates based on rotifer count just prior to harvest
2. For 165 micron long L-Type rotifers with a mass of 0.18g dry weight/million rotifers
3. Feed continuously for best results
Batch Production Systems
| Rotifer density (per ml) | ||||||
|---|---|---|---|---|---|---|
| Rotigrow Nanno (feed per day per million rotifers) | Day 0 | Day 1 | Day 2 | Day 3 | Rotifer Harvest (per liter of feed) | Growth Rate (per day) |
| 1.3 ml | 500 | 706 | 996 | 1,406 | 320 million | 41% |
| 1.8 ml | 500 | 795 | 1,264 | 2,010 | 320 million | 59% |
| 2.6 ml | 500 | 910 | 1,656 | 3,013 | 320 million | 82% |
NOTES: 1. Feed rates based on rotifer counts at the beginning of the day
2. For 165 micron long L-Type rotifers with a mass of 0.18g dry weight/million rotifers
3. Feed days 0, 1 and 2. Feed continuously for best results
Fatty Acids
| 12:0 | Lauric acid | 0.0 | 18:3n6 | GLA | 0.6 |
| 14:0 | Myristic acid | 2.2 | 18:3n3 | ALA | 0.3 |
| 16:0 | Palmitic acid | 21.5 | 18:4n3 | SDA | 0.1 |
| 16:1n7 | Palmitoleic acid | 3.3 | 20:3n6 | DGLA | 0.3 |
| 16:2n4 | Hexadecadienoic | 0.2 | 20:4n6 | ARA | 3.6 |
| 18:0 | Stearic acid | 0.4 | 20:5n3 | EPA | 30.3 |
| 18:1n9 | Oleic acid | 18.0 | 22:5n3 | DPA (n-3) | 0.0 |
| 18:2n6 | LA | 4.7 | 22:6n3 | DHA | 0.0 |
NOTE: fatty acid units after "profile" units are mg/g d.w.
Summary
| DHA : EPA : ARA | 0 : 1.0 : 0.12 |
|---|---|
| EPA + DHA | 30 mg/g d.w. (Biomass) |
| Total HUFAs, 20:3n3 | 35 mg/g d.w. (Biomass) |
| Total fatty acids | 105 mg/g d.w. (Biomass) |
Rotifer Composition
Essential Fatty Acids and Total Lipids
Typical profiles of rotifers grown on RotiGrow Plus and enriched using N-Rich High PRO, a protein-rich feed, and N-Rich PL Plus, a phospholipid-rich feed.
| Rotigrow Nanno | 3-hour enrichment N-Rich PL Plus | 6 to 8 hour enrichment N-Rich PL Plus & High PRO | Cod eggs | Cobia eggs | |
|---|---|---|---|---|---|
| Protein | 68% | 66% | 64% | 79% | n/a |
| DHA | 0.0 | 30 | 38 | 33 | 42 |
| EPA | 20 | 13 | 12 | 18.6 | 13.5 |
| ARA | 2.8 | 2.5 | 2.5 | 2.2 | 3.4 |
| HUFAs | 32 | 50 | 57 | 56.2 | 68 |
| Total fatty acids | 8.9% | 13.0% | 14.0% | 13.2% | 22% |
| Polar lipids | 56% | 55% | 55% | 71% | n/a |
| Sterols | 5% | 4.2% | 4.3% | 9.8% | n/a |
| Free fatty acids | 5% | 5.1% | 4.9% | 5.4% | n/a |
NOTE: fatty acid units after "profile" units are mg/g